Infection with human papillomavirus(HPV) is considered to be the principal causal agent in the development of the uterine cervical cancer. To detect HPV types prevalent in carcinoma of the uterine cervix, a type-specific, sensitive polymerase
chain
reaction(PCR)-based assay for HPV types 16, 18, and 33 was applied to 40 cervical carcinomas(39 squamous cell carcinomas and 1 adenocarcinoma), 6 cases of cervical intraepithelial neoplasia(CIN), and 18 samples of cervicitis and histologically
normal
cervix. PCR was done using 2 sets of L1 primer of HPV-16 and 18, 1 set of E2/E1 primer of HPV-33, and 3 sets of E6 primer of HPV-16, 18, 33. The amplified product was analyzed directly on the basis of the size of the ethidium bromide-stained band
visible after agarose gel electrohoresis, which was confirmed by Southern blot hybridization.
@ES The results were as follows :
@EN 1. As expected, the combined incidence of the common high-risk genital HPVs(types 16, 18 and 33) was high in carcinomas(82.5%) and CIN III(66.7%), low in CIN 1 (33.3%), and nonexistent in the normal controls.
2. Bu using L1 primers and E1/E2 primers in 46 CINs and cervical carcinomas, HPV DNA was detected in 36(78.%3%), which were 65.2%(30/46) for HPV 16, 8.7%(4/46) for HPV 33, 6.5%(3/46) for HPV-18, and only 1 case of mixed infection of HPV-16 and
HPV-18
was detected. All normal cervices and cervicitis tissues were negative for HPV 16, 18 and 33. By using E6 primers, 35(76.1%) cases were positive for HPV DNA, which were 67.4%(31/46) for HPV-16, 4.3%(2/46) each for HPV-18 and HPV-33. HPV 16 is the
most
common type present in squamous cell carcinoma.
3. There was no difference in detection rates of HPV DNA according to patients' age in cervical carcinoma(p=0.188).
4. There were good correlation between the results obtained by PCR using L1 or E1/2 primer and those by E6 primer. The concordance rates were 93.8%(60/64) for HPV-16, 98.4% for HPV-18, and 96.9% for HPV-33.
5. There was no difference in detection rates of HPV DNA according to clinical stage in cervical carcinoma(p=0.2).
6. The detection rate of HPV DNA in case of serum SCC level under 10ng/ml was 100%(17/17), which was higher than those in cases of serum SCC levels of 10ng/ml or more. The detection rate was significantly decreased as serum SCC became high in
level(p=0.012).
7. There was no difference in detection rates of HPV DNA according to tumor size in cervical carcinoma(p=0.743).
8. The reccurrence rate of patients with HPV-16 containing tumors was 28.6%(8/28), which was 3.4 times more than that of patients without HPV-16, but it was not statistically significant(p=0.162). The survival rate of patients with HPV-16
containing
tumors was 82.1%,f which was not different from from that of patients without HPV-16 containing tumrs(p=0.405).
In conclusion, papillomavirus, especially HPV-16, played some role in the development of cervical cancer. There was no association between the HPV infection and the clinical characteristics including prognosis. However, in contrast to other
studies, HPV
type 18 and 33 were not the common type of HPV. Perhaps more case should be analyzed.
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